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Stimulus‐Dependent Leukotriene Release From Human Basophils: A Comparative Study of C5a and Fmet‐leu‐phe
Author(s) -
MacGlashan Donald,
Warner Jane
Publication year - 1991
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.49.1.29
Subject(s) - basophil , peptide , leukotriene , histamine , n formylmethionine leucyl phenylalanine , biology , leukotriene c4 , granulocyte , calcium , formyl peptide receptor , anaphylatoxin , biophysics , immunology , biochemistry , chemotaxis , pharmacology , medicine , receptor , complement system , immune system , immunoglobulin e , asthma , antibody
Previous studies of human basophil mediator release have noted that the bacterial peptide fmet‐leu‐phe and the anaphylatoxin C5a induce comparable levels of histamine release while only fmet peptide induces leukotriene release. Since 5‐lipoxygenase metabolism of arachidonic acid is calcium dependent, we examined the characteristics of the human basophil [Ca ++ ] i response which follows its activation by either fmet peptide or C5a. While the peak [Ca ++ ] i response was essentially identical for these two stimuli, fmet peptide induced a prolonged increase in [Ca ++ ] i while C5a stimulated only a transient increase in [Ca ++ ] i that was essentially over within 2 minutes of adding the stimulus. Simultaneous addition of EDTA with fmet peptide revealed the two phases of the [Ca ++ ] i response and demonstrated that leukotriene release was dependent on an elevated [Ca ++ ] i level in the 2–5 minutes following challenge. Enhancement of leukotriene release induced by C5a by agents such as staurosporine and interieukin‐3 also produced a [Ca ++ ] i kinetic curve which resembled fmet piptide. Single cell studies of the [Ca ++ ] i response could detect no subpopulations of cells which responded preferentially to fmet peptide or C5a, eliminating the possibility that the ability of fmet peptide to induce leukotriene was a result of its action on a functionally distinct population of basophils.