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Avian Inflammatory Macrophage Function: Shifts in Arachidonic Acid Metabolism, Respiratory Burst, and Cell‐Surface Phenotype During the Response to Sephadex
Author(s) -
Golemboski K.A.,
Whelan J.,
Shaw S.,
Kinsella J.E.,
Dietert R.R.
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.48.6.495
Subject(s) - superoxide , biology , arachidonic acid , respiratory burst , stimulation , macrophage , sephadex , thromboxane , thromboxane b2 , metabolism , microbiology and biotechnology , endocrinology , biochemistry , medicine , immunology , in vitro , enzyme , platelet
The avian inflammatory response to intraperitoneal (i.p.) Sephadex injection produces macrophages which display characteristics of an increasingly activated state over time. We examined elicited chicken peritoneal exudate cells (PECs) with respect to superoxide anion production, arachidonic acid metabolism and cell surface la and transferrin receptor (TfR) expression from 4 to 96 h after i.p. stimulation. Avian PECs showed the highest level of superoxide release when harvested just 4 h after injection, and did not produce PGE 2 or 6‐keto PGF 1α . Early (4‐h) PECs produced elevated amounts of thromboxane as compared to later (42‐h) macrophages. Expression of both la and TfR increased between 4 and 24 h after Sephadex stimulation; TfR remained elevated through 96 h, but la declined after 42 h. Some aspects of chicken macrophage regulation of superoxide anion, thromboxane release, and surface antigen expression are in contrast with those reported for mouse macrophages.