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Effect of Retinoids on the Release and Gene Expression of Tumor Necrosis Factor‐α in Human Peripheral Blood Monocytes
Author(s) -
Turpin Jim,
Mehta Kapil,
Blick Mark,
Heater Jeane P.,
LopezBerestein Gabriel
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.48.5.444
Subject(s) - tumor necrosis factor alpha , cytolysis , lipopolysaccharide , biology , in vitro , retinoic acid , endogeny , microbiology and biotechnology , monocyte , endocrinology , immunology , cell culture , biochemistry , cytotoxicity , genetics
The effect of retinoic acid (RA) and retinol (ROH) on the release of tumor necrosis factor (TNF) by human peripheral blood monocytes (HPBM) was determined. HPBM were cultured for various periods of time in either 5% complete (cAB) or delipidized (DLS) AB serum. TNF release (L929 cytolytic assay) in the presence of cAB occurred during the first 3 days of in vitro culture. Delipidization of AB serum completely inhibited the lipopolysaccharide (LPS)‐induced release of TNF by HPBM. Addition of RA (0.5 μM) to DLS restored LPS‐induced TNF release by HPBM, and supplementation with ROH (1.0 μM) resulted in release of TNF‐like activity, but only after 3 days of in vitro culture. The maintenance of TNF release by the addition of exogenous RA after 3 days of in vitro culture suggested that depletion of endogenous RA was partially responsible for loss of TNF‐like activity. The levels of endogenous TNF protein and mRNA were not influenced by delipidization of serum and were found to be similar to those of HPBM cultured in the presence of AB serum. TNF protein and mRNA were undetectable in HPBM ROH‐treated cell lysates, although cytolytic activity was observed in culture supernatants. These results suggest that retinoids are required for the release of cytolytic factors from HPBM and that non‐TNF cytolytic factors may be released by these cells at different stages of maturation.