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Activated Neutrophils Induce Erythrocyte Immunoglobulin Binding and Membrane Protein Degradation
Author(s) -
Weiss Douglas J.,
Murtaugh Michael P.
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.48.5.438
Subject(s) - biology , antibody , erythrocyte membrane , immunology , microbiology and biotechnology , immunoglobulin g , membrane protein , degradation (telecommunications) , membrane , biochemistry , telecommunications , computer science
Neutrophil‐induced alterations in feline erythrocytes were investigated to elucidate the pathogenesis off the erythrocyte destruction associated with inflammatory disease. Incubation of erythrocytes with zymosan‐activated neutrophils, at target:effector concentrations of 50:1, resulted in immunoglobulin binding to the erythrocyte membrane and degradation of membrane proteins. Vitamin E and superoxide dismutase/catalase attenuated the immunoglobulin binding. Proteolysis was not prevented by addition of antioxidants or protease inhibitors to the erythrocyte:neutrophil coculture and was not reproduced with chemical oxidation or calcium loading of erythrocytes.