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Effects of Adenosine on Inositol 1,4,5‐Trisphosphate Formation and Intracellular Calcium Changes in Formyl‐Met‐Leu‐Phe‐Stimulated Human Neutrophils
Author(s) -
Walker Blair A.M.,
Hagenlocker Brian E.,
Douglas Vonda K.,
Ward Peter A.
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.48.3.281
Subject(s) - adenosine , superoxide , intracellular , inositol , biology , calcium , calcium in biology , inositol phosphate , inositol trisphosphate , medicine , endocrinology , adenosine triphosphate , biochemistry , receptor , enzyme
In the presence of adenosine, formyl‐Met‐Leu‐Phe‐stimulated human neutrophils show a greatly diminished production of superoxide anion. Analysis of changes in levels of intracellular calcium revealed that the immediate increase (occurring within seconds) in intracellular calcium following addition of formyl‐Met‐Leu‐Phe is not affected by the presence of adenosine, although there are significantly lower intracellular calcium levels during the late phase (occurring 1–4 min after addition of formyl‐Met‐Leu‐Phe). Consistent with these findings is the fact that adenosine does not affect the production of inositol 1,4,5‐trisphosphate in formyl‐Met‐Leu‐Phe‐stimulated neutrophils. These data suggest that the profound inhibitory effects of adenosine on superoxide responses in formyl‐Met‐Leu‐Phe‐stimulated neutrophils may be related to an action of adenosine occurring late in the sequence of events of signal transduction.