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Suppression of Late Phase Enhanced Vascular Permeability in Rats by Selective Depletion of Neutrophils With a Monoclonal Antibody
Author(s) -
Sekiya Sakae,
Yamashita Takao,
Sendo Fujiro
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.48.3.258
Subject(s) - zymosan , monoclonal antibody , vascular permeability , neutrophile , infiltration (hvac) , edema , biology , immunology , antibody , inflammation , pharmacology , medicine , endocrinology , in vitro , biochemistry , physics , thermodynamics
We investigated the role of neutrophils in increased vascular permeability by a selective reduction of neutrophils using a monoclonal antibody, RP‐3. An intraperitoneal injection of RP‐3 not only selectively depleted peripheral blood neutrophils, but prevented the neutrophil infiltration to the tissues. Proteose peptone, zymosan, and BCG induced three different types of inflammatory edema, showing the early phase only, early plus late phase, and the late phase only, respectively. Only the late phase response of zymosan and BCG was inhibited by a depletion of neutrophils by RP‐3, though the early phase response induced by proteose peptone and zymosan was not affected. Reconstitution of neutrophil‐depleted rats by in situ infection of these cells restored the inflammatory edema induced by BCG, depending upon the number of neutrophils injected.