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Constitutive Expression of Interleukin‐7 mRNA and Production of IL‐7 by a Cloned Murine Thymic Stromal Cell Line
Author(s) -
Sakata Tsuneaki,
Iwagami Shoji,
Tsuruta Yuji,
Teraoka Hiroshi,
Tatsumi Yoichi,
Kita Yasumichi,
Nishikawa ShinIchi,
Takai Yasuyuki,
Fujiwara Hiromi
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.48.3.205
Subject(s) - biology , stromal cell , clone (java method) , microbiology and biotechnology , messenger rna , cell culture , t cell , interleukin 4 , cytokine , immunology , gene , cancer research , immune system , genetics
Mouse interleukin‐7 (IL‐7) cDNA was cloned from mouse thymic stromal cell clone MRL104.8a using a polymerase chain reaction (PCR) technique and expressed in COS‐7 cells. The resulting recombinant interleukin‐7 (rlL‐7) supported the proliferation of mouse antigen‐specific helper T cell (Th) clone 9–16 in the absence of IL‐2 and antigen as well as mouse pre‐B cell line DW34. It was also found that high levels of the mRNA for IL‐7 were constitutively expressed in the MRL104.8a cells, and a potent amount of IL‐7 was produced in its culture supernatant. These results provide the evidence for constitutive expression of IL‐7 mRNA and for production of IL‐7 by thymic stromal cells that have a critical role in intrathymic T cell development. The results are discussed in the context of the functional and molecular relationship between IL‐7 and the previously described cytokines produced by thymic stromal cells.