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Activated Macrophages Use Different Cytolytic Mechanisms to Lyse a Virally Infected or a Tumor Target
Author(s) -
LeBlanc Paul A.,
Heath Lucie S.,
Um HongDuck
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.48.1.1
Subject(s) - biology , cytolysis , lysis , vesicular stomatitis virus , population , tumor necrosis factor alpha , microbiology and biotechnology , cytotoxicity , virus , immunology , biochemistry , in vitro , demography , sociology
Murine bone‐marrow‐culture‐derived‐macrophages can be differentially activated to lyse either vesicular stomatitis virus infected BALB/c3T3 cells or the tumor target P815. Macrophages were activated in a manner so that they could lyse both targets. The ability of this activated population to lyse either target type was differentially inhibited by varying the assay conditions. The lysis of P815 targets was more sensitive to inhibition by the proteinase inhibitor N‐p‐tosyl‐L‐lysine chloromethyl ketone than was the lysis of virally Infected cells. On the other hand, reduction of the concentration of glucose in the assay medium, which inhibits the production of oxygen metabolytes by the hexose monophosphate shunt, or the addition of anti‐tumor necrosis factor (anti‐TNF) serum were able to decrease the lysis of virally infected targets but not P815 targets. Thus, the observed differences in the lysis of these two targets were due to both the activation state of the macrophages and the differential susceptibility of the targets to different effector mechanisms.