z-logo
Premium
Comparative Abilities of Various Metabolites of Vitamin D to Protect Cultured Human Macrophages Against Tubercle Bacilli
Author(s) -
Crowle Alfred J.,
Ross Elise J.
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.47.6.545
Subject(s) - tubercle , biology , bacilli , microbiology and biotechnology , vitamin , bacteria , biochemistry , genetics
Vitamin D metabolites have several biologic functions besides the best‐known one of controlling mineral metabolism, one of which may be protection against tuberculosis. The chemical structures of the metabolites govern their functions. The most potent metabolite for regulating calcium metabolism is 1,25(OH) 2 ‐vitamin D 3 (1,25‐D 3 ). This metabolite also is able to protect cultured human monocytes and macrophages (MP) against tubercle bacilli (TB). To determine whether these two functions are connected, 14 other analogs or metabolites of vitamin D were compared with 1,25‐D 3 for ability to protect cultured MP against TB. Blood‐derived MP from 18 different donors were used in 70 experiments. The MP were infected with TB, then incubated for 7 days in medium containing 4 μg/ml of metabolite or synthesized analog. Growth of TB in the MP was measured by colony‐forming unit counts from samples of lysed MP at 0, 4, and 7 days after infection. The metabolites, none of which inhibited TB in the absence of MP, varied from unprotective to bacteriostatic in the MP. Four of them were nearly as protective as 1,25‐D 3 , and the metabolite 25S,26(OH) 2 ‐vitamin D 3 was consistently more protective. An analog synthetically designed for maximum ability to promote cell differentiation was unprotective. There was no correlation between metabolite ability to protect and known ability to stimulate calcium mobilization. These results suggest that antituberculosis protection of human MP by vitamin D metabolites or analogs can be separated from their functions of inducing cell differentiation and controlling mineral metabolism.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here