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Inhibition of Neutrophil and Eosinophil Chemotactic Responses to PAF by the PAF‐Antagonists WEB‐2086, L‐652,731, and SRI‐63441
Author(s) -
Håkansson Lena,
Venge Per
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.47.5.449
Subject(s) - eosinophil , chemotaxis , biology , immunology , asthma , receptor , biochemistry
Abstract The influence of the three PAF‐antagonists WEB‐2086, L‐652,731, and SRI‐63441 on the chemotactic response of neutrophil and eosinophil granulocytes to PAF was investigated. When the PAF‐antagonists were added to the cell suspension that was exposed to a gradient of PAF, WEB‐2086 and SRI‐63441 at the concentration of 10 ‐6 mol/litre inhibited ( P <.01) the neutrophil and eosinophil chemotactic response to 10 ‐8 and 10 ‐9 mol PAF per litre; at the concentration of 5 x 10 ‐6 mol/litre, WEB‐2086 and SRI‐63441 also inhibited ( P <.02) the response to 10 ‐7 mol PAF per litre. Under the same conditions L‐652,731 at the concentration of 5 x 10 ‐6 mol/litre inhibited ( P <.01) the eosinophil chemotactic response to 10 ‐8 and 10 ‐9 mol PAF per litre. The inhibition of the chemotactic response to PAF by the three PAF‐antagonists was specific, since the chemotactic response to C5f, f‐MLP, and LTB 4 was not affected by WEB‐2086, L‐652,731, or SRI‐63441, neither was the chemokinetic migration induced by albumin.