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Repopulation of Macrophages in Popliteal Lymph Nodes of Mice After Liposome‐Mediated Depletion
Author(s) -
Delemarre F.G.A.,
Kors N.,
Kraal G.,
Rooijen N.
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.47.3.251
Subject(s) - biology , liposome , lymph , repopulation , microbiology and biotechnology , immunology , cancer research , pathology , stem cell , haematopoiesis , biochemistry , medicine
Macrophages lining the subcapsular sinus (SCS) and those located in the medulla of popliteal lymph nodes (PLN) of mice were eliminated after subcutaneous (s.c.) injection of dichloromethylene diphosphonate (CI 2 MDP)‐containing liposomes. No effect of liposome‐entrapped CI 2 MDP could be seen on nonphagocytic cells, e.g., interdigitating cells (IDC) and B‐ and T‐lymphocytes. One month after injection the eliminated subsets of macrophages were still absent. After 2 mo a small number of macrophages had reappeared along the SCS and in the medulla of the PLN of a few animals. Complete repopulation of the PLN with macrophages was observed only after 5 mo. This extremely long repopulation time could be shortened drastically by local administration of Freund's complete adjuvant (FCA). A small number of macrophages reappeared along the SCS and in the medulla 1 wk after FCA and after 3 wk the repopulation of the PLN with macrophages was complete. Such rapid repopulation of macrophages was not achieved after s.c. injection of paraffin oil or paratyphoid vaccine. These results indicate that the normal rate of influx of mononuclear phagocytes into the PLN is low, but that it can be sped up after administration of FCA.