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Priming Effect of Muramyl Peptides for Induction by Lipopolysaccharide of Tumor Necrosis Factor Production in Mice
Author(s) -
Parant Monique,
Parant Francine,
Vinit MarieAntoinette,
Jupin Claude,
Noso Yoshihiro,
Chedid Louis
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.47.2.164
Subject(s) - muramyl dipeptide , lipopolysaccharide , tumor necrosis factor alpha , biology , necrosis , pharmacology , immunology , ratón , toxicity , cytotoxic t cell , microbiology and biotechnology , immune system , medicine , in vitro , biochemistry , genetics
Lipopolysaccharide‐induced necrosis of grafted tumors was potentiated by several hydrophilic and lipophilic muramyl dipeptide (MDP) derivatives administered a few hours prior to small amounts of lipopolysaccharide (LPS) in spite of low titers of induced circulating tumor necrosis factor (TNF). However, pretreatment with MDP derivatives did increase the level of TNF in the blood of mice challenged by a greater dose of LPS. The TNF amount in 2 h postendotoxin mouse serum reached a peak when the glycopeptide had been given 6 h before the challenge, being approximately 100‐fold above that obtained in unprimed mice. The cytotoxic activity in mouse serum was inhibited by rabbit antibodies raised against recombinant mouse TNF. Although there exists a toxic synergism between BCG or MDP and endotoxin, the effect of certain MDP derivatives was not related to an increased susceptibility to the toxicity of LPS.