Pertussis Toxin Inhibits the FMLP‐lnduced Membrane Association of Protein Kinase C in Human Neutrophils
Author(s) -
Christiansen Niels Obel
Publication year - 1990
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.47.1.60
Subject(s) - pertussis toxin , superoxide , protein kinase c , ionomycin , protein kinase a , biology , chromosomal translocation , lyn , microbiology and biotechnology , phorbol , kinase , g protein , biochemistry , signal transduction , enzyme , proto oncogene tyrosine protein kinase src , intracellular , gene
Abstract The effect of pertussis toxin on N‐formyl‐methionyl‐leucyl‐phenylalanine (FMLP), phorbol 12‐myristate 13‐acetate (PMA), ionomycin, and A23187‐induced translocation of protein kinase C and superoxide generation was studied in human neutrophils. Pertussis toxin was shown to inhibit FMLP‐induced superoxide generation in parallel with an inhibition of protein kinase C translocation. In contrast, no effect of pertussis toxin was demonstrated upon superoxide generation or protein kinase C translocation in PMA‐, A23187‐, or ionomycin‐stimulated cells. In that superoxide generation and protein kinase C translocation were inhibited in parallel, it is concluded that a G‐protein‐dependent cascade is involved in the FMLP‐induced activation of protein kinase C, and this cascade may be equivalent to the pathway inducing superoxide generation.