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Quantitation of the Host Cell Infiltration Kinetics of the Nonimmunogenic Colon 26 Tumor by Multiparameter Flow Cytometry
Author(s) -
Sneed Rosie A.,
Stevenson Anita P.,
Stewart Carleton C.
Publication year - 1989
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.46.6.547
Subject(s) - in vivo , flow cytometry , infiltration (hvac) , biology , immunogenicity , kinetics , cell , pathology , cancer research , immune system , immunology , medicine , materials science , physics , microbiology and biotechnology , genetics , quantum mechanics , composite material
In this study, we examined the kinetics of host cell infiltration into the nonimmunogenic Colon 26 tumor. We found that 1 x 10 4 cells were required to produce tumors in 100% of mice. The in vivo doubling time was 42.5 h, and a barely palpable tumor contained 8 x 10 6 cells. No evidence of concomitant immunity was found. The number of host cells infiltrating the in vivo tumors increased at the same rate as the number of tumor cells, but averaged only 22% of total cells. Cycling T lymphocytes were present in the host cell infiltrate of this tumor. In addition, approximately 50% of in vivo Colon 26 cells were Thy‐1.2 positive. The observed characteristic of low immunogenicity makes it a useful murine model for studying human malignant tumors.