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Quantitation of Conjugate Formation Between Human Polymorphonuclear Leukocytes and Antibody‐Coated Target Cells by Flow Cytometry: The Role of Fc Receptor and LFA‐1 Antigen
Author(s) -
Kessel Kok P.M.,
Strijp Jos A.G.,
Tol Marijke E.,
KatsRenaud Henny J.,
Thijssen Rik M.W.M.,
Fluit Ad C.,
Verhoef Jan
Publication year - 1989
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.46.5.467
Subject(s) - monoclonal antibody , flow cytometry , antibody , microbiology and biotechnology , biology , fluorescein isothiocyanate , antiserum , fc receptor , antigen , conjugate , epitope , immunology , fluorescence , mathematical analysis , physics , mathematics , quantum mechanics
Abstract The specific binding of human polymorphonuclear leukocytes (PMN) to antibody‐coated target cells was characterized by flow cytometry. PMN were labeled with phycoerythrin‐E (PE) via a granulocyte‐specific monoclonal antibody (leu‐M1) and mixed with fluorescein isothiocyanate‐labeled K562 tumor cells sensitized with rabbit antiserum. Specific conjugates were formed as analyzed by two‐color fluorescence in a flow cytometer. The formation of stable conjugates was dependent on initiation of contact, temperature, time, and antiserum concentration. Studies with inhibitors implicate that microfilaments, but not microtubules, Ca 2+ , Mg 2+ , or energy‐dependent processes were a prerequisite for binding of PMN to the antibody‐coated target cells. No conjugates were formed when uncoated target cells were used or when the experiment was performed in the presence of protein A, indicating that binding was specifically mediated through Fc receptors (FcR). Monoclonal antibodies against the FcRII and FcRIII were used to address the role of these receptors in conjugation. One of the two anti‐FcRIII antibodies and an anti‐FcRII antibody effectively prevented conjugation. A monoclonal antibody directed against the common β‐chain of the adhesion molecule family and a combination of antibodies against the α‐chain of LFA‐1 and Mo‐1 also blocked conjugation when target cells were sensitized under suboptimal conditions. The antibody against the β‐chain also diminished killing of antibody‐coated K562, as measured by chromium release when included in the cytotoxicity assay. These results indicate that flow cytometry permits accurate quantitation and characterization of the binding between PMN and antibody‐coated target cells, which in principle, can be prevented by monoclonal antibodies against surface receptors. Binding is primarily established by both the FcRII and FcRIII. Adhesion‐associated molecules on the PMN surface contribute to optimal binding.