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Functions of Purified Mouse Neutrophils Isolated From Gelatin Sponges
Author(s) -
Middleton Marjorie M.,
Campbell Priscilla A.
Publication year - 1989
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.46.5.461
Subject(s) - ficoll , sponge , biology , chemotaxis , phagocytosis , gelatin , microbiology and biotechnology , population , neutrophile , immunology , inflammation , in vitro , biochemistry , medicine , peripheral blood mononuclear cell , botany , receptor , environmental health
When sterile gelatin sponges are implanted under the skin of a mouse and retrieved 6 hr later, approximately 10 6 neutrophils per mouse, in a 98–99% pure population, may be retrieved by simply squeezing and rinsing the sponges. These neutrophils behave similarly to peritoneal exudate neutrophils in chemotaxis and phagocytosis assays, but are bacteriostatic rather than bactericidal. The sponge method yields substantially more neutrophils than could be obtained by exsanguinating the mouse and isolating blood neutrophils, and a 5‐fold purer population than is normally obtained by passage of peritoneal exudate cells over Ficoll. In addition, sponge‐elicited neutrophils may be ready for use within a half hour after removal from the mouse, without being exposed to osmotic shock or Ficoll.