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Human Neutrophils Activated by Interferon‐γ and Tumour Necrosis Factor‐α Kill Entamoeba histolytica Trophozoites In Vitro
Author(s) -
Denis Michel,
Chadee Kris
Publication year - 1989
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.46.3.270
Subject(s) - entamoeba histolytica , microbiology and biotechnology , biology , interferon gamma , in vitro , cytokine , tumor necrosis factor alpha , immunology , chemotaxis , receptor , biochemistry
The interaction between cytokine‐activated human neutrophils and Entamoeba histolytica trophozoites was studied as well as the mechanism(s) involved. Treatment of neutrophils with rlFN‐γ alone allowed them to kill 30% of E. histolytica trophozoites; however, rlFN‐γ and rTNF‐α pretreatments in combination increased neutrophil killing to 70%. In the absence of direct contact between neutrophils and amebae, rlFN‐γ‐treated neutrophils were shown to kill 70% of amebae, and rlFN‐γ‐ and rTNF‐α‐treated neutrophils killed 97% of amebae. Neutrophil enhancement of amebicidal activity following cytokine treatments was correlated with Increased neutrophil resistance to amebic contact‐dependent killing and was shown to be 73% H 2 O 2 dependent.