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Change of Membrane Fluidity of Rat Neutrophils Accompanying Escherichia coli Inoculation
Author(s) -
Masuda Midori,
Komiyama Yutaka,
Nishikado Hiroyuki,
Kuriki Hideki,
Egawa Hiroshi,
Murata Kenjiro
Publication year - 1989
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.46.2.169
Subject(s) - membrane fluidity , escherichia coli , biology , ethylenediaminetetraacetic acid , incubation , cytochalasin b , microbiology and biotechnology , membrane , biochemistry , in vitro , chemistry , organic chemistry , chelation , gene
Membrane fluidity of rat neutrophils was studied following Escherichia coli inoculation, and characteristic changes were observed. Membrane fluidity was assessed by the excimer‐forming lipid technique using pyrenedecanoic acid and flow cytometry and expressed as the fluorescence intensity ratios of excimer and monomer pyrenedecanoic acid (l E /L M ratio). High l E /l M ratios indicated high membrane fluidity. The IE/I m ratio of rat neutrophils (0.50 ± 0.048) increased after E . coli inoculation, reaching a maximum of almost 1.00 after 10‐20 min and then returning to its starting value. Intravenous injection of heat‐killed E . coli or E . coli‐conditioned culture supernatants into rats induced a rapid increase of l E /l M ratios, which returned to initial levels after 20 min. The effect on membrane fluidity of in vitro neutrophil incubation with E . coli , heat‐killed E . coli , or E . coli conditioned culture supernatants was similar to that observed in vivo. Addition of 5 mM ethylenediaminetetraacetic acid (EDTA) did not affect neutrophil membrane fluidity. Addition of either 5 μg/ml cytochalasin B or 10 ‐5 M colchicine did not directly affect neutrophil membrane fluidity but did block the change observed following incubation with bacteria.