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Selective Potentiation of Lymphocyte‐Derived Macrophage Chemotactic Factor Release in Complete Freund's Adjuvant‐Treated Guinea Pigs
Author(s) -
Sakata Kenmei,
Muramoto Kazuhiro,
Hirashima Mitsuomi,
Hayashi Hideo
Publication year - 1989
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.46.2.144
Subject(s) - biology , long term potentiation , chemotaxis , immunology , macrophage , guinea pig , lymphocyte , adjuvant , freund's adjuvant , lymphokine , immune system , endocrinology , in vitro , biochemistry , receptor
Dinitrophenol (DNP)‐ovalbumin(OA)‐induced tissue macrophage reaction in sensitized guinea pigs is enhanced by treatment with complete Freund's adjuvant (CFA). The enhancement of the reaction may be due to the increased production of a T‐lymphocyte‐derived macrophage chemotactic factor (LDMCF) because treatment of animals with CFA potentiates antigen‐ and concanavalin A(ConA)‐induced release of LDMCF activity from spleen cells of the CFA‐treated animals in vitro. This potentiating effect by CFA seems to be ascribed to the release of an adherent‐cell‐derived soluble factor from the CFA‐treated animals. The adherent cell‐derived factor, LDMCF‐potentiating factor (LDMCF‐PF), preferentially potentiates the release of LDMCF activity but not of eosinophil chemotactic activity from antigen‐ or Con‐A‐stimulated T lymphocytes. Protein synthesis is required for release of LDMCF‐PF. Molecular weight of LDMCF‐PF activity is assumed to be about 10,000‐20,000. LDMCF‐PF activity is sensitive to trypsin, to neuraminidase, and also to alkalinity at pH 11, suggesting that LDMCF‐PF is a glycoprotein. The present study provides one explanation for the enhanced macrophage reaction in delayed‐type hypersensitivity reactions.