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Chemiluminescence in a Macrophage Cell Line Modulated by Biological Response Modifiers
Author(s) -
Tosk Jeff,
Lau Benjamin H.S.,
Lui Paul,
Myers R. Craig,
Torrey Robert R.
Publication year - 1989
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.46.2.103
Subject(s) - biology , zymosan , macrophage , cell culture , chemiluminescence , respiratory burst , lysis , in vitro , biological response modifiers , phagocytosis , microbiology and biotechnology , phagocytic cell , interferon , immunology , immune system , biochemistry , chemistry , genetics , organic chemistry
We have studied a murine macrophage cell line, J774, and found these cells capable of a zymosan‐triggered chemiluminescent oxidative burst. Such activity was enhanced by preincubation with Corynebacterium parvum (CP), bacillus Calmette‐Guerin, and Iipopolysaccharide (LPS). Under similar conditions, CP and LPS were shown to enhance J774‐mediated tumor cell lysis. We have also demonstrated that murine interferon α + β rendered J774 cells more sensitive to the actions of CP and LPS. These results indicate that J774 cells may be useful for the in vitro evaluation of biological response modifiers as well as the study of oxygen radical production by macrophages.