Premium
Stimulation of Granulocytic Cell Iodination by Pine Cone Antitumor Substances
Author(s) -
Unten Senwa,
Sakagami Hiroshi,
Konno Kunio
Publication year - 1989
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.45.2.168
Subject(s) - stimulation , peripheral blood mononuclear cell , myeloperoxidase , biology , cell culture , retinoic acid , iodine , fibroblast , cell , microbiology and biotechnology , microsome , biochemistry , medicine , endocrinology , immunology , chemistry , in vitro , genetics , organic chemistry , inflammation
Antitumor substances (Fractions VI and VII) prepared from the NaOH extract of pine cone significantly stimulated the iodination (incorporation of radioactive iodine into an acid‐insoluble fraction) of human peripheral blood adherent mononuclear cells, polymorphonuclear cells (PMN), and human promyelocytic leukemic HL‐60 cells. In contrast, these fractions did not significantly increase the iodination of nonadherent mononuclear cells, red blood cells, other human leukemic cell lines (U‐937, THP‐1, K‐562), human diploid fibroblast (UT20Lu), or mouse cell lines (L‐929, J774.1). iodination of HL‐60 cells, which were induced to differentiate by treatment with either retinoic acid or tumor necrosis factor, were stimulated less than untreated cells. The stimulation of iodination of both PMN and HL‐60 cells required the continuous presence of these fractions and was almost completely abolished by the presence of myeloperoxidase inhibitors. The stimulation activity of these fractions was generally higher than that of various other immunopotentiators. Possible mechanisms of extract stimulation of myeloperoxidase‐containing cell iodination are discussed.