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Infection of a Murine T‐Cell Line With a Retrovirus Carrying c‐ myc Decreases the Interleukin‐2 Cell Dependence by a Nonautocrine Mechanism
Author(s) -
Shapiro Ilya M.,
Winberg Gösta,
Stefanescu Irina,
Wigzell Hans,
Menezes José
Publication year - 1988
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.44.6.559
Subject(s) - biology , retrovirus , cell culture , microbiology and biotechnology , interleukin 2 , transduction (biophysics) , endogeny , complementary dna , interleukin 4 , virology , cell , interleukin , gene , cytokine , immunology , genetics , biochemistry
A murine Interleukin‐2 (IL‐2)‐dependent T‐cell line, CTLL‐2, was infected with a retrovirus carrying the mouse c‐ myc cDNA and the Tn‐5 neogene. Transduced cells were selected in the presence of Geneticin sulfate (G418); these cells were shown to express both the endogenous and the transduced c‐ myc genes. The IL‐2 requirement of these cells was then found to be significantly reduced. The cells did not express IL‐2 mRNA nor did they produce an activity myogenic for CTLL‐2 cells. This suggests that the reduction of IL‐2 dependence observed following retroviral transduction of c‐ myc is caused by a nonautocrine mechanism.