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Establishment and Characterization of Factor‐Dependent Macrophage Cell Lines
Author(s) -
Ohki Kazunori,
Nagayama Ariaki
Publication year - 1988
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.44.6.465
Subject(s) - biology , macrophage , cell culture , microbiology and biotechnology , factor (programming language) , immunology , genetics , in vitro , computer science , programming language
Three macrophage cell lines from bone marrow cells of C3H/HeN mice were isolated by successive transfer of the cells in culture with L‐cell‐conditioned medium (LCM) or WEHI‐3 cell‐conditioned medium (WEHI‐3CM). These cell lines, which express Fc receptors, are involved in Fc‐mediated phagocytosis and possess nonspecific esterase activity. Two (BDM‐1 and BDM‐2) of three cell lines show dependency for growth on either macrophage colony‐stimulating factor (M‐CSF) (CSF‐1) or granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) and do not respond to interleukin 3 (IL‐3). The third clone (BDM‐3) proliferates in response to IL‐3 as well as to GM‐CSF and weakly responds to M‐CSF and to interleukin 4 (IL‐4). GM‐CSF, in combination with the suboptimal concentration of M‐CSF, acted synergistically on the proliferation of BDM‐1 cells. The tumor‐promoting phorbol diester, 12‐o‐tetradecanoyl‐phorbol‐13‐acetate (TPA) also acted synergistically with the three CSFs (IL‐3, GM‐CSF, and M‐CSF) to stimulate the proliferation of BDM‐1 cells. The synergistic effect was observed when cells were pretreated with TPA and subsequently stimulated with IL‐3. The calcium ionophore A23187 enhanced the proliferation of BDM‐1 cells costimulated with TPA and IL‐3. These factor‐dependent macrophage cell lines should be useful for studying signal transduction mechanisms in the regulation of cell growth.