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Graunulocyte‐Macrophage Colony Stimulating Factor (GM‐CSF) and Macrophage Colony Stimulating Factor (CSF‐1) Synergize to Stimulate Progenitor Cells With High Proliferative Potential
Author(s) -
Falk Lydia A.,
Vogel Stefanie N.
Publication year - 1988
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.44.5.455
Subject(s) - progenitor cell , macrophage colony stimulating factor , biology , bone marrow , macrophage , colony stimulating factor , immunology , granulocyte macrophage colony stimulating factor , growth factor , population , andrology , microbiology and biotechnology , cytokine , stem cell , haematopoiesis , medicine , in vitro , biochemistry , receptor , environmental health
Abstract Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland The ability to expand a population of bone marrow progenitors capable of forming macrophage colonies of high proliferative potential (HPP‐CFC) was achieved using a culture system and signal requirements not previously shown to support the growth of HPP‐CFC. Using bone marrow cells from untreated animals (not 5‐FU‐treated), culture conditions designed primarily for the detection of GM‐CFC or M‐CFC progenitors, and a more stringent criteria for HPP‐CFC colony size (≥ 2 mm diameter), HPP‐CFC progenitor expansion was demonstrated following simultaneous addition of rGM‐CSF and CSF‐1 to bone marrow cultures. Examination of the sequence and temporal requirements for rGM‐CSF and CSF‐1 addition necessary for the development of HPP‐CFC‐like colonies revealed that addition of the second factor could be delayed for up to 5 days and still result in the development of significant numbers of HPP‐CFC colonies. Based on a comparison with human spleen cell conditioned medium (HSCM) and interleukin 1 (rIL 1), as sources of “synergistic activity” (SA) for the development of HPP‐CFC‐like colonies in combination with CSF‐1, the combination of GM‐CSF and CSF‐1 appears to represent a novel pathway for stimulating the expansion of HPP‐CFC progenitors with high proliferative potential.