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Superoxide Anion Production in Glass‐Adherent Polymorphonuclear Leukocytes and Its Relationship to Calcium Movement
Author(s) -
RebutBonneton C.,
Bailly S.,
Pasquier C.
Publication year - 1988
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.44.5.402
Subject(s) - superoxide , extracellular , calcium , stimulation , neutrophile , intracellular , biology , n formylmethionine leucyl phenylalanine , calcium in biology , biochemistry , microbiology and biotechnology , endocrinology , medicine , in vitro , enzyme
The production of superoxide anion (O) was measured in relation to 45 Ca movement in glass‐adherent polymorphonuclear leukocytes (PMNs), and the results were compared with those obtained by ourselves and others on PMNs in suspension. In adherent PMNs, O production stimulated by N‐formyl‐methionyl‐leucyl‐phenylalanine (FMLP) was of rapid onset and short duration; this was also true of PMNs in suspension. However, O production was insensitive to the concentration of extracellular calcium. Both in adherent and non‐adherent PMNs, O production stimulated with phorbol myristate acetate (PMA) had a latency time and was of long duration. In adherent PMNs, pretreatment with PMA potentiated the FMLP‐induced O production by lengthening its duration without changing its initial rate. In adherent PMNs, (10 ‐10 ‐10 ‐7 M) FMLP induced a fast but transient dose‐dependent increase in 45 Ca within 1 min, whereas PMA only released 45 Ca about 5 min after its addition to the cell culture medium. Pretreatment of PMNs with 10 or 100 ng/ml PMA for 3 min before stimulation by 10 ‐7 M FMLP reduced the 45 Ca efflux observed with FMLP alone. We conclude that O production by adherent PMNs cannot simply be related to Ca 2+ movement. In comparison with non‐adherent cells, adherence seemed to interfere with the characteristics of both calcium and O generation, probably by modifying the cytoskeleton.