Premium
Effects of Retinoids on Macrophage Function and IL‐1 Activity
Author(s) -
Dillehay D.L.,
Walia A.S.,
Lamon E.W.
Publication year - 1988
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.44.5.353
Subject(s) - biology , macrophage , function (biology) , immunology , microbiology and biotechnology , biochemistry , in vitro
The effects of three retinoids, all‐ trans ‐retinoic acid (RA), 13‐ cis ‐retinoic acid (cRA), and N‐(4‐hydroxyphenyl) ratinamide (4‐HPR), on macrophage function were evaluated. In vitro, RA, cRA, and 4‐HPR caused a greater than twofold increase in phagocytosis of IgG‐sensitized bovine erthrocytes (IgG‐ORBC) by a mouse macrophage cell line (RAW). Significant increases in phagocytosis were produced by retinoid concentrations as low as 2 x 10 ‐10 M. RA also significantly increased phagocytosis of IgG‐sensitized ORBC by BALB/c peritoneal macrophages In vitro. The ability of RAW macrophages to bind IgG‐ORBC was significantly increased by 10 ‐6 to 10 ‐14 M RA. The potentiation of myogenic responses of spleen cells to Con A and PWM by RA was relatively independent of macrophage function, i.e., splenocytes that were macrophage‐depleted were responsive to the potentiating effects of RA. The effects of retinoids on T‐cell‐dependent B‐cell mitogenesis induced by PWM appeared not to be dependent on their previously reported capacity to alter prostaglandin synthesis. Treatment of spleen cells with 10 ‐6 M indomethacin did not abolish the potentiating effects of RA. However, RA in a dose‐dependent fashion increased IL‐1 activity at the level of the target T‐cell. The greatest potentiation of IL‐1 activity was at 10 ‐8 M RA. These results show that retinoids can modulate macrophage function at two different levels: potentiation of phagocytosis and potentiation of IL‐1 activity at the level of the T‐cell.