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Phorbol Myristate Acetate Induced Oxidation of 2′,7′‐Dichlorofluorescin by Neutrophils From Patients With Chronic Granulomatous Disease
Author(s) -
Hassan Nassef F.,
Campbell Donald E.,
Douglas Steven D.
Publication year - 1988
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.43.4.317
Subject(s) - chronic granulomatous disease , biology , phorbol , granulomatous disease , immunology , disease , microbiology and biotechnology , pathology , protein kinase c , medicine , signal transduction
The oxidative metabolic burst of stimulated human polymorphonuclear leukocytes (PMNs) has been evaluated by the measurement of oxygen consumption, chemiluminescence, and oxygen radicals (O 2 ‐ , H 2 O 2 ,OH ‐ ) derived from activation of the hexose monophosphate shunt (HMPS). PMNs from patients with chronic granulomatous disease (CGD) are shown to lack functional NADPH oxidase and undetectable oxygen radical generation. However, using single cell analysis by flow cytometry and 2′,7′‐dichlorofluorescin (DCFH) oxidation by H 2 O 2 , significant DCFH oxidation by the PMA stimulated CGD PMNs was observed. Furthermore, 1mM potassium cyanide enhanced DCFH oxidation by control and CGD PMNs. DCFH oxidation by cells from an obligate heterozygous mother of an X‐linked CGD patient was intermediate. These observations suggest that a PMA induced oxidase enzyme is present in CGD cells.