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Regulation by Interferon γ of Function in the Acute Monocytic Leukemia Cell Line, THP‐1
Author(s) -
Gaffney E.V.,
Lingenfelter S.E.,
Koch G.A.,
Lisi P.J.,
Chu C.W.,
Tsai S.C.
Publication year - 1988
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.43.3.248
Subject(s) - thp1 cell line , monocytic leukemia , acute monocytic leukemia , biology , cell culture , incubation , radioimmunoassay , leukemia , acute leukemia , antigen , microbiology and biotechnology , immunology , in vitro , endocrinology , biochemistry , genetics
THP‐1 is an acute monocytic leukemia cell line which acquires phenotypic and functional monocytoid‐like features following incubation with mezerein. The current study concerned the modulation of these features by rIFNγ. rIFNγ induces the time‐dependent enhancement of HLA‐DR expression in the presence or absence of mezerein but has no effect on the expression of Leu‐M1, Leu‐M2, or Leu‐M3 antigens. CSF‐1 production following mezerein activation was reduced by incubation in the presence of 10 3 and 10 4 units/ml rIFNγ. This was confirmed through both biological assays with mouse bone marrow cells and an indirect ELISA. In contrast, the concentration of growth inhibitory activity in conditioned medium was increased by rIFNγ. A small but significant increase in IL‐1β concentration in conditioned medium was detected using a sensitive double‐antibody ELISA and a radioimmunoassay. The results infer that the functional characteristics of this leukemia cell line are modulated by rIFNγ in a manner qualitatively similar to that reported for IFNγ treated normal monocytes.