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Distinct Subpopulations of Elicited Human Macrophages in Peritoneal Dialysis Patients and Women Undergoing Laparoscopy: A Study on Peroxidatic Activity
Author(s) -
Bos H.J.,
Bronswijk H.,
Helmerhorst T.J.M.,
Oe P.L.,
Hoefsmit E.C.M.,
Beelen R.H.J.
Publication year - 1988
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.43.2.172
Subject(s) - peritoneal dialysis , biology , laparoscopy , peritoneum , medicine , immunology , general surgery , anatomy
The endogenous peroxidatic activity (PA) pattern of peritoneal macrophages from 24 continuous ambulatory peritoneal dialysis (CAPD) patients and from five healthy women undergoing laparoscopy was studied. In general, the macrophages showed two different PA patterns in vivo: exudate and negative macrophages. However, two of 24 CAPD patients showed resident macrophages, the first described in vivo in man. Since, in general, the examined human peritoneal macrophages are exudate and PA‐negative, this suggests, in accordance with the animal model system, that a chronic sterile inflammation exists in the peritoneal cavity of CAPD patients and healthy women undergoing laparoscopy. After 2 hr culture, blood monocytes and peritoneal macrophages transformed into cells with the characteristics of exudate‐resident and resident macrophages, so isolation procedures that Include short periods of culture can change the developmental stage of human monocytes and macrophages.