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Characterization of Surface Markers of Continuously Growing Murine Resident Macrophages
Author(s) -
Muller Christian D.,
Lombard Yves,
Bartholeyns Jacques,
Poindron Philippe,
Schuber Francis
Publication year - 1988
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.43.2.165
Subject(s) - biology , phagocytosis , mannose receptor , receptor , macrophage , in vitro , antigen , microbiology and biotechnology , spleen , bovine serum albumin , fc receptor , mannose , immunology , biochemistry
Conditions have been described which allow an in vitro indefinite multiplication of differentiated murine macrophages (Lombard et al: Biol Cell 53, 219, 1985). R. and MAY‐1 ceil lines, which were obtained, respectively, from mouse (Balb/c) spleen and resident peritoneal macrophages, have been further characterized. They present at their surface, besides the Mac‐1 antigen and Fc‐receptor, a mannose receptor which was characterized for its binding properties. This receptor Is responsive for a specific phagocytosis of mannosylated particles, i.e., mannosylated latex beads or oil droplets containing mannosylated bovine serum albumin. Moreover, R and MAY‐1 ceils present an ectoenzyme profile (NAD + glycohydrolase and nucleotide pyrophosphatase) similar to those of the corresponding resident macrophages.