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Expression of Ia Antigen in Colonies of Bone Marrow‐Derived Macrophages
Author(s) -
Yen ShingErh,
Beelen Robert H.J.,
Walker William S.
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.42.6.697
Subject(s) - biology , antigen , bone marrow , lymphokine , progenitor cell , microbiology and biotechnology , population , macrophage , immunology , in vitro , stem cell , genetics , demography , sociology
Cells in colonies of culture‐derived mouse and rat bone marrow macrophages were examined for membrane la antigen to determine if the steady‐state expression of the antigen was restricted to macrophages derived from a distinct population of progenitor cells. Multiple subcultures of macrophages derived from single soft‐agar colonies were tested for 1a + cells on three different days of culture. About one‐half of the colonies gave rise to subcultures that never contained 1a + cells, about 40% yielded subcultures that contained some la+ cells on at least 2 of the 3 assay days, and about 10% of the colonies produced subcultures that contained 1a‐bearing cells on all 3 assay days. Thus, when cultures were assayed at any one time for their content of 1a‐bearing cells, the results raised the possibility of phenotypically distinct subpopulations of progenitors. However, sequential analyses of the subcultures revealed the variable expression of la on cells from at least one‐half of the colonies. We conclude that, under steady‐state conditions, the presence of la antigen on bone marrow‐derived macrophages is not clonally restricted. That a T‐cell‐derived lymphokine induced la antigen on essentially all the cells in most of the colonies of macrophages confirms their potential to express the antigen.