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Inhibition of Neutrophil Shape Change by an Inhibitor of Chemotaxis
Author(s) -
Donabedian Haig,
Sawyer Thomas,
Senitzer David
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.42.5.510
Subject(s) - chemotaxis , biology , peptidoglycan , chemotaxis assay , shape change , peripheral blood mononuclear cell , microbiology and biotechnology , biophysics , in vitro , biochemistry , receptor , enzyme
Human mononuclear cells exposed to staphylococcal peptidoglycan in serum‐free culture rapidly produce an inhibitor of neutrophil chemotaxis which we have previously described. We found that this inhibitor of chemotaxis has its most potent effect on the inhibition of neutrophil shape change from a spherical to a polarized configuration. In order to quantify this shape change inhibition, we developed an assay using flow cytometric techniques. Neutrophils exposed to a chemoattractant simultaneously change their shape and decrease their forward angle light scattering intensity (δFLS) with a correlation coefficient of 0.886 (p < 0.001). In 51 experiments, neutrophils pretreated with the inhibitor of chemotaxis decreased their FLS by only 6.8 ±1.3 channels, while neutrophils pretreated with medium or control culture supernatants decreased theirs by 26.4 ±1.9 and 20.5 ± 3.0 channels respectively (p < 0.001). The factor which causes inhibition of shape change was indistinguishable from the inhibitor of chemotaxis by physical properties and chromatography. We conclude that this inhibitor of chemotaxis may act by inhibiting a physiologic step at or before shape change.