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Pertussis Toxin Blocks the Inhibitory Effects of Calcitonin on Cyclic AMP Accumulation in Stimulated Cultured Human Monocytes
Author(s) -
Stock John L.,
Coderre James A.
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.42.5.504
Subject(s) - pertussis toxin , biology , inhibitory postsynaptic potential , toxin , calcitonin , cholera toxin , medicine , microbiology and biotechnology , endocrinology , pharmacology , signal transduction , g protein
Abstract Surface stimulation of fresh or cultured human mononuclear cells by latex particles causes an increase in the accumulation of cyclic AMP that is inhibited by preincubation with calcitonin (CT). Preincubation of cultured monocytes with 500 ng/ml pertussis toxin totally blocks the inhibitory effects of CT at low concentrations of this hormone. The effects of pertussis toxin are dose‐related and eliminated by boiling the toxin. Similar preincubations with cholera toxin have no significant effects on subsequent inhibition of surface‐stimulated cyclic AMP by CT. Membranes prepared from cultured human monocytes contain a 41,000‐dalton protein that is ADP‐ribosylated by pertussis toxin and may be the inhibitory guanine nucleotide regulatory protein (N) mediating this inhibition.