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Rat Monocytes in a Model of Combined Injury Express the OX8 Antigen
Author(s) -
Kaffenberger W.,
Gruber D.F.,
MacVittie T.J.
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.42.3.181
Subject(s) - peripheral blood mononuclear cell , antigen , population , immunology , cell sorting , biology , microbiology and biotechnology , ficoll , andrology , flow cytometry , medicine , genetics , in vitro , environmental health
We have analyzed peripheral blood mononuclear cell preparations from a rat model of combined injury (CI) [whole‐body irradiation (500 cGy 60 Co) followed by a thermal injury (20% body surface area, dorsal, scald burn)] for the expression of OX8 antigens. Ficoll‐separated mononuclear fractions were labeled with monoclonal antibodies MRC OX8, MRC OX19, W3/13 HLK, or W3/25 for flow cytometric analysis. Combined‐injury trauma resulted in decreased mononuclear cells to 6% of normal. This effect was due to the rapid decrease in radiosensitive lymphocytes from 83% to 10%. The relative numbers of monocytes increased from a normal 13% to 70% at day 4 after CI. Labeling of cells with OX8 after CI shifted to a population which was significantly larger in volume than normal lymphocytes. At the same time the mean fluorescence intensity of OX8‐positive cells was considerably reduced. With the use of a F(ab) fragment of OX8 as a probe, these results could be partially explained as unspecific binding of the whole molecule of OX8 to Fc receptors expressed by activated monocytes. But double‐labeling and cell‐sorting experiments also revealed the expression of OX8 antigens by a subset of OX8 + / OX19‐ monocytes after CI.