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Defective Production of Reactive Oxygen Intermediates by Tumor‐Associated Macrophages Exposed to Phorbol Ester
Author(s) -
Ghezzi Pietro,
Erroi Annalaura,
Acero Raffaella,
Salmona Mario,
Mantovani Alberto
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.42.1.84
Subject(s) - superoxide , zymosan , biology , reactive oxygen species , phorbol , tetradecanoylphorbol acetate , macrophage , biochemistry , in vitro , protein kinase c , enzyme
Macrophages were isolated from poorly immunogenic metastatic sarcomas (mFS6 and MN/MCA1) of C57BL/6 origin. Tumor‐associated macrophages (TAM) showed little release of superoxide when exposed to phorbol myristate acetate. When exposed to a phagocytic stimulus (zymosan), TAM released appreciable amounts of superoxide. TAM had a lower number of specific binding sites for phorbol esters than resident or caseinate‐elicited peritoneal macrophages, but had normal NADPH‐cytochrome C reductase. The tumor environment, possibly through previously demonstrated products of neoplastic cells, may influence the functional status of in situ macrophages and, thus, impair host anti‐tumor and anti‐microbial defense mechanisms.