Premium
Expression of an Activation Antigen, Mo3e, Associated With the Cellular Response to Migration Inhibitory Factor by HL‐60 Promyelocytes Undergoing Monocyte‐Macrophage Differentiation
Author(s) -
Todd Robert F.,
Bury Martin J.,
Liu David Y.
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.41.6.492
Subject(s) - biology , monocyte , ibmx , macrophage migration inhibitory factor , macrophage , cellular differentiation , microbiology and biotechnology , promyelocyte , cholera toxin , monoclonal antibody , antigen , macrophage activating factor , in vitro , immunology , forskolin , antibody , biochemistry , endocrinology , cytokine , myeloid , gene
HL‐60 promyelocytic cells acquire the surface expression of the Mo3e antigenic determinant after exposure to PMA or compounds that raise intracellular concentrations of cyclic AMP (dibutyryl cyclic AMP or a combination of cholera toxin and IBMX). The expression of Mo3e by these stimulated HL‐60 cells coincides with the development of features of monocyte‐macrophage differentiation (characteristic morphology, nonspecific esterase activity, and respiratory burst activity). During in vitro monocyte‐macrophage differentiation, HL‐60 cells become responsive to migration inhibitory factor (MIF); the MIF responsiveness of differentiated HL‐60 cells is blocked by anti‐Mo3e monoclonal antibody. These findings further support the relationship between the expression of Mo3e and the cellular response to MIF.