Premium
Complement Component C3 Secretion by Mouse Macrophage‐Like Cell Lines
Author(s) -
Goodrum Kenneth J.
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.41.4.295
Subject(s) - secretion , biology , lipopolysaccharide , cell culture , macrophage , cycloheximide , microbiology and biotechnology , spleen , lymphokine , cell , immunology , in vitro , biochemistry , protein biosynthesis , antigen , genetics
Secretion of complement component C3 by the mouse macrophage‐like cell lines PU5‐1.8, J774A.1, RAW264.7, and P388D1 was measured using an enzyme‐linked immunosorbent assay for mouse C3. All cell lines secreted antigenically detectable C3 with the relative secreted C3/10 6 cells/24 h ranked as J774A.1 > P388D1 PU5‐1.8 > > RAW264.7. C3 secretion was enhanced two‐ to fourfold in cultures of all cell lines when treated with lipopolysaccharide, streptococcal cell walls, or lymphokine‐containing supernatant fluids of mitogen‐stimulated spleen cells. A differential induction of C3 synthesis and secretion was indicated since secreted lysozyme and total cellular protein were not elevated in a manner comparable to C3. The relative inducibility of cell lines for C3 secretion in either lipopolysaccharide‐ or cell wall‐treated cells could be ranked as PU5‐1.8 > P388D1 > J774A.1 > RAW264.7. C3 secretion was inhibited by cycloheximide or hydrocortisone. Mouse macrophage‐like cell lines retain baseline and inducible C3 synthetic activities as do normal macrophages and can serve as homogeneous cultures in which to study regulation of complement biosynthesis.