Premium
Candidacidal Activity of Monocyte‐Derived Human Macrophages: Relationship Between Candida Killing and Oxygen Radical Generation by Human Macrophages
Author(s) -
Sasada Masataka,
Kubo Akemi,
Nishimura Toshiro,
Kakita Tokio,
Moriguchi Toshinori,
Yamamoto Kohkichi,
Uchino Haruto
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.41.4.289
Subject(s) - biology , monocyte , macrophage , microbiology and biotechnology , phagocytosis , reactive oxygen species , phagocyte , oxygen , immunology , biochemistry , in vitro , chemistry , organic chemistry
Freshly isolated human monocytes ingested and killed Candida albicans , and generated O ‐ 2 H 2 O 2 and .OH efficiently. When monocytes were cultured in vitro, these cells transformed into macrophages. Cultured monocytes retained their ingestive activity but lost their candidacidal activity almost completely after day 3. The release of O ‐ 2 by monocytes decreased slightly with culture and that of .OH was markedly decreased on day 3 of culture. The activity of myeloperoxidase in the monocytes decreased with culture. These results suggested that the loss of candidacidal activity is due to the decrease of .OH generation and myeloperoxidase activity in cultured monocytes.