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Respiratory Burst of Normal Human Eosinophils
Author(s) -
Petreccia David C.,
Nauseef William M.,
Clark Robert A.
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.41.4.283
Subject(s) - respiratory burst , biology , zymosan , stimulation , respiratory system , immunology , granulocyte , eosinophil , eosinophilia , chemotaxis , endocrinology , biochemistry , in vitro , anatomy , receptor , asthma
Oxidative metabolism of eosinophils has generally been studied in cells from patients with eosinophilia. We isolated eosinophils with purity of >95% from normal donors. Oxygen metabolism of eosinophils and neutrophils was compared using O 2 production as a measure of the stimulus‐induced respiratory burst. During 10 min of stimulation, using phorbol myristate acetate (PMA) or opsonized zymosan (OPZ), eosinophils produced two to three times as much O 2 as did neutrophils. Continuous kinetic analysis was used to measure the latency period and maximal rate of O 2 production. Eosinophils had a shorter latency period and greater rate of O 2 production than did neutrophils at all concentrations of PMA. With OPZ stimulation, O 2 production rates by both cells were similar. The latency periods were similar with high concentrations of OPZ but at limiting amounts of OPZ eosinophils had a longer latency period than did neutrophils. In addition, the kinetic assay demonstrated that the respiratory burst in eosinophils was more sustained than in neutrophils. These results indicate substantial differences in the oxidative burst of eosinophils and neutrophils with respect to activation, capacity, and regulation. These distinctive features of O 2 production by eosinophils may be important in host defense against metazoan parasites or in tissue injury during inflammation.