Premium
Suppressive Effect of Interferon‐β on Development of Tumoricidal Activity in Mouse Macrophages
Author(s) -
Pace Judith L.,
MacKay Robert J.,
Hayes Mark P.
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.41.3.257
Subject(s) - biology , interferon , immunology , macrophage , interferon gamma , microbiology and biotechnology , cancer research , virology , cytokine , in vitro , biochemistry
The suppressive effect of IFN‐alpha and IFN‐beta on the induction of tumoricidal activity in mouse bone marrow‐derived macrophages was investigated. Macrophages incubated for 24 hr with IFN‐beta developed lower levels of cytolytic activity when stimulated with IFN‐gamma and LPS, in comparison with macrophages pretreated with medium. The suppressive effect was dependent on the pretreatment dose of IFN‐beta over a concentration range of 1 to 1,000 U/ml. Analysis of IFN‐gamma dose response curves of IFN‐beta treated macrophages showed that these cells were less sensitive to IFN‐gamma. The suppressive effects were fully neutralized by an antiserum to IFN‐alpha/beta. Prostaglandins were apparently not involved in this process since the addition of indomethacin to IFN‐beta treated macrophages did not prevent the loss of responsiveness to activating stimuli. In contrast to the results obtained with IFN‐alpha and IFN‐beta, macrophages pretreated with IFN‐gamma did not develop lower levels of cytolytic activity when again stimulated with IFN‐gamma and LPS. These observations provide evidence for a potentially important negative regulatory role for IFN‐alpha and IFN‐beta in macrophage activation for tumor cell killing.