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1,25‐Dihydroxyvitamin D 3 Stimulates Interieukin‐2 Production by a T Cell Lymphoma Line (MLA‐144) Cultured in Vitamin D‐Deficient Rat Serum
Author(s) -
Haverty Thomas,
Haddad John G.,
Neilson Eric G.
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.41.2.177
Subject(s) - biology , medicine , endocrinology , cell culture , calcitriol , vitamin d and neurology , vitamin , receptor , calcitriol receptor , microbiology and biotechnology , biochemistry , genetics
A lymphocyte T celt line (MLA‐144), which constitutively secretes interieulin‐2 (IL‐2), was shown to express receptors for 1,25‐diydroxyvitamin D 3 (1,25(OH) 2 D 3 ). The proliferation of an IL‐2‐dopondent cell line (HT‐2) in response to supernatant! from MLA‐144 cells was employed as an index of IL‐2 production by MLA‐144 cells. IL‐2 production was two fold higher from MLA‐144 cells cultured in 2% vitamin D‐deficient rat serum compared to 10% fetal calf serum (FCS). The addition of 1,25(OH) 2 D 3 at 10 ‐15 Mor 10 ‐11 M augmented IL‐2 production by MLA‐144 cells in vitamin D‐deflcient rat serum, but not in fetal calf serum. At 10 ‐7 M 1,25(OH) 2 D 3 there was inhibition of IL‐2 production by MLA‐144 cells In either vitamin D‐deflcient serum or FCS. There was no effect of 1,25(OH) 2 D 3 , added directly to HT‐2 celis. Monoclonal antibody to the IL‐2 receptor competitively inhibited the proliferation of HT‐2 cells in response to MLA‐144 supernatants, suggesting that it was IL‐2 from the MLA‐144 supernatants which influenced HT‐2 proliferation. Our findings demonstrate biphasic does effects of 1,25(OH) 2 D 3 on rymphokine secretion. The use of vitamin D deficient rat serum allowed us to demonstrate the effects of 1,25(OH) 2 D 3 in the physiologic and subphysiologic range.