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Preferential Loss of DNA Polymerase α Following Suppression of Replicative DNA Synthesis of Guinea Pig Macrophages by the Immunostimulants Muramyl Dipeptide or Lipopolysaccharide
Author(s) -
Haraguchi Tokuko,
Nagao Shigeki,
Tanaka Atsushi,
Nagano Hiroshi
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.41.2.170
Subject(s) - muramyl dipeptide , biology , dna synthesis , dna , lipopolysaccharide , guinea pig , polymerase , dna replication , microbiology and biotechnology , immunology , biochemistry , genetics , immune system
Oil‐induced guinea pig peritoneal exudate macrophages were found to incorporate 3 H‐thymidine into trichloroacetic acid‐insoluble fraction. In pulie labeling experiments, the moorporsted 3 H‐thymiome was detected in short fragmetns or DNVA, wnicn corresponded to the Okazaki fragments. These results indicate that the observed thymidine incorporation is due to nuclear DNA replication but not DNA repair. The observed DNA synthesis of the macrophages was remarkably suppressed when the cells were cuftured in a presence of muramyl dipeptide (MDP) or bacterial lipoporysaccharide (LPS). The significant decrease of DNA polymerase a activity was found in the celts treated with MDP or LPS. in contrast, the activity of polymerase β was not at all affected by the same treatment.