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Differentiation of Rat Bone Marrow Cells Into Macrophages Under the Influence of Mouse L929 Cell Supernatant
Author(s) -
BoltzNitulescu George,
Wiltschke Christoph,
Holzinger Christoph,
Fellinger Alois,
Scheiner Otto,
Gessl Alois,
Förster Othmar
Publication year - 1987
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.41.1.83
Subject(s) - biology , bone marrow , microbiology and biotechnology , antigen , macrophage , progenitor cell , monoclonal antibody , major histocompatibility complex , cell , immunology , immunofluorescence , cellular differentiation , antibody , stem cell , in vitro , biochemistry , gene
Bone marrow cells (BMC) flushed from femora of Lewis rats were cultured in Dulbecco's modification of Eagle's medium supplemented with mouse L929 cell supernatant as a source of colony‐stimulating factor (CSF). Differentiation of macrophage progenitor cells into macrophages (Mø) and expression of various markers were kinetically assessed. The proportion of Mø increases from approximately 4% in freshly isolated BMC to 100% after 7‐8 days of cell culture. These cells, termed bone marrow cell‐derived macrophages (BMDMø), adhere to and spread on plastic surface; exhibit Mø morphology; stain intensely for nonspecific esterase; are able to phagocytose latex particles, IgG‐sensitized erythrocytes, and C3‐coated red cells; and express receptors for IgG and C3. A subpopulation of BMDMø expresses MHC class II antigens as demonstrated by immunofluorescence using MRC OX6 and MRC OX17 monoclonal antibodies which recognize antigens coded in the I‐A or I‐E subregion of the MHC, respectively. Collectively, our results show that supernatant from mouse L929 cells supports and is continuously required for proliferation and differentiation of rat BMC into typical Mø, and suggest that mouse CSF cross‐reacts with the putative receptor on rat Mø.