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Enhanced Luminol‐Dependent Chemiluminescence of Stimulated Rat Alveolar Macrophages by Pretreatment With Alveolar Lining Material
Author(s) -
Webb Deborah S.A.,
Jeska Edward L.
Publication year - 1986
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.40.1.55
Subject(s) - chemiluminescence , luminol , phagocytosis , alveolar macrophage , chemistry , in vitro , macrophage , pulmonary alveolus , biochemistry , microbiology and biotechnology , chromatography , biology
Previous reports indicate that the in vitro bactericidal activity of rat alveolar macrophages (AM) is dependent on the lipid fraction (ALM‐L) of the alveolar lining material (ALM). The present study demonstrates that luminol‐dependent chemiluminescence of stimulated rat AM is increased when rat AM are preincubated in the ALM or in the ALM‐L. Evidence is presented that oxidation of the unsaturated lipids is responsible for the increase. In addition to pretreatment with the ALM, cells were also pretreated in commercial preparations of several lipids found to be present in the ALM. Preincubation in these lipids produced a significant increase in the luminol‐dependent chemiluminescence response. However, when a saturated lipid, dipalmitoyl phosphatidycholine, was used no increase was found. Pretreatment in ALM did not increase the nitro blue tetrazolium dye reduction by the AM, nor was the phagocytosis of latex beads by the AM altered by the addition of the ALM.