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Intravital imaging of eosinophils: Unwrapping the enigma
Author(s) -
Nguyen William N.T.,
Jacobsen Elizabeth A.,
Finney Constance A.M.,
Colarusso Pina,
Patel Kamala D.
Publication year - 2020
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.3hr0220-396r
Subject(s) - biology , intravital microscopy , cognitive science , neuroscience , psychology , in vivo , microbiology and biotechnology
Eosinophils are traditionally associated with allergic and parasitic inflammation. More recently, eosinophils have also been shown to have roles in diverse processes including development, intestinal health, thymic selection, and B‐cell survival with the majority of these insights being derived from murine models and in vitro assays. Despite this, tools to measure the dynamic activity of eosinophils in situ have been lacking. Intravital microscopy is a powerful tool that enables direct visualization of leukocytes and their dynamic behavior in real‐time in a wide range of processes in both health and disease. Until recently eosinophil researchers have not been able to take full advantage of this technology due to a lack of tools such as genetically encoded reporter mice. This mini‐review examines the history of intravital microscopy with a focus on eosinophils. The development and use of eosinophil‐specific Cre (EoCre) mice to create GFP and tdTomato fluorescent reporter animals is also described. Genetically encoded eosinophil reporter mice combined with intravital microscopy provide a powerful tool to add to the toolbox of technologies that will help us unravel the mysteries still surrounding this cell.