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Local Proliferation and Extrahepatic Recruitment of Liver Macrophages (Kupffer Cells) in Partial‐Body Irradiated Rats
Author(s) -
Bouwens L.,
Knook D.L.,
Wisse E.
Publication year - 1986
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.39.6.687
Subject(s) - bone marrow , biology , zymosan , kupffer cell , macrophage , monocyte , population , mononuclear phagocyte system , cell growth , endocrinology , medicine , pathology , immunology , in vitro , biochemistry , environmental health , genetics
The relative significance of local proliferation and extrahepatic recruitment of Kupffer cells was investigated by partial‐body irradiation before the induction of macrophage hyperplasia by zymosan. There was no difference in growth of the Kupffer cells population between nonirradiated rats and rats irradiated with the liver shielded, whereas irradiation of the liver with the rest of the body (bone marrow) shielded resulted in strong inhibition of growth (–61%). Splenectomy combined with bone marrow irradiation inhibited growth to a lesser extent as compared to liver irradiation (–38%). Monocyte and other leukocyte numbers were strongly reduced in peripheral blood and their accumulation in the liver was completely prevented by bone marrow irradiation. Our results demonstrate that local proliferation of resident Kupffer cells represents the predominant source for their increased number during hyperplasia.