Premium
Human Monocyte Production of Interleukin‐1: Parameters of the Induction of Interleukin‐1 Secretion by Lipopolysaccharides
Author(s) -
Newton R.C.
Publication year - 1986
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.39.3.299
Subject(s) - lipopolysaccharide , monocyte , secretion , biology , interleukin , microbiology and biotechnology , activator (genetics) , salmonella , enterobacteriaceae , cytokine , escherichia coli , bacteria , biochemistry , immunology , receptor , gene , genetics
The role of lipopolysaccharide (LPS) as an activator of human monocyte interleukin‐1 (IL‐1) synthesis and secretion has been examined in this study. The results demonstrate that when blood monocytes are prepared under low endotoxin conditions, they do not spontaneously secrete IL‐1 activity. When cells are exposed to LPS extracted from different bacterial species, there is variation seen in the potency, with LPS from Salmonella species being the most potent in inducing IL‐1 activity from human monocytes. This material is tenfold more potent than LPS obtained from three different strains of Escherichia coli and 10,000‐fold more potent than material obtained from two other bacterial species. Detoxified endotoxins are inefficient activators for IL‐1 secretion. When monocytes are exposed to LPS, there is a rapid rise in the level of IL‐1 activity detected. Activity can be detected in cell lysates after 1 hr with appreciable accumulation seen over the first 6 hr of culture. This is accompanied by IL‐1 release into the surrounding medium after 2 hr of culture with subsequent accumulation. Monocyte synthesis of IL‐1 activity appears to be sensitive to fg/ml levels of Salmonella minnesota LPS, while appreciable secretion of this activity by monocytes requires pg/ml levels.