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Effect of Platelet‐Activating Factor on Human Polymorphonuclear Leukocyte Enhancement of Chemiluminescence and Antibody‐Dependent Cellular Cytotoxicity
Author(s) -
Mossmann Horst,
Bamberger Uwe,
Velev Boris A.,
Gehrung Marina,
Hammer Dietrich K.
Publication year - 1986
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.39.2.153
Subject(s) - antibody dependent cell mediated cytotoxicity , respiratory burst , platelet activating factor , chemiluminescence , cytotoxicity , platelet , diltiazem , receptor , biology , extracellular , granulocyte , stimulation , phagocytosis , calcium , immunology , biophysics , microbiology and biotechnology , biochemistry , chemistry , endocrinology , in vitro , organic chemistry
The effect of platelet‐activating factor on human granulocytes was determined by luminol‐dependent chemiluminescence (CL), antibody‐dependent cellular cytotoxicity (ADCC), and rosette assay. An activation of the respiratory burst could be measured by CL and be shown to depend on the presence of extracellular calcium. This direct stimulation of PMN was proved to be inhibited by oxygen radical scavengers as well as by the calcium channel blocker diltiazem. Furthermore, the presence of PAF enhanced the activation of PMN via Fc‐ or C3b‐receptors, as demonstrated by CL and ADCC. On the other hand, no influence on the rosette‐forming capacity of PMN could be detected. The results support the concept of the import role of PAF in inflammatory processes.