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Continuous Expression of I‐A Antigen by Peritoneal Macrophages From Mice Resistant to Mycobacterium bovis (Strain BCG)
Author(s) -
Johnson Sarah C.,
Zwilling Bruce S.
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.38.5.635
Subject(s) - biology , antigen , mycobacterium bovis , microbiology and biotechnology , macrophage , monoclonal antibody , population , salmonella , listeria monocytogenes , antibody , immunology , mycobacterium tuberculosis , in vitro , bacteria , tuberculosis , medicine , pathology , biochemistry , genetics , environmental health
Monoclonal antibodies were used to monitor the expression of I‐A antigen on the surface of macrophages obtained from mice immunized to Mycobacterium bovis (strain BCG). Unlike the transient nature of I‐A expression by macrophages from Listeria‐injected mice, peritoneal macrophages from mice injected 28 days previously with 10 4 BCG expressed I‐A continuously. The continued expression was not due to the presence of antigen or of contaminating lymphocytes. When we compared the kinetics of I‐A expression from different strains of mice, the continuous expression of I‐A correlated with the genetic resistance of the mice to BCG. Macrophages from mice that were resistant to BCG expressed I‐A continuously, while macrophages from BCG susceptible mice expressed I‐A transiently. Injection of resistant mice with Salmonella typhimurium did not result in the induction of a population of macrophages that expressed I‐A continuously. This suggests that the Bcg gene may not be the same as that responsible for resistance to Salmonella (Ity) or Leishmania (Lsh).