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An Inhibitory Factor Against Monocyte Spreading in the Sera of Patients With Systemic Lupus Erythematosus
Author(s) -
Baba Toru,
Ochiya Takahiro,
Baba Akiko,
Yamaguchi Koji,
Yaoita Hideo,
Uyeno Kenichi
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.38.4.481
Subject(s) - monocyte , sephadex , inhibitory postsynaptic potential , stimulation , immunology , biology , lupus erythematosus , endocrinology , medicine , antibody , biochemistry , enzyme
The effect of the sera of patients with systemic lupus erythematosus (SUE) on monocyte function was studied using cell spreading as an indicator. Monocyte spreading induced by exogenous stimuli was shown to be inhibited by SLE sera. Gel filtration of SLE sera on Sephadex G‐200 revealed that the factor responsible for this inhibition had a molecular weight of about 50,000. Pretreatment of monocytes with the inhibitory factor led to suppression of cell spreading induced by subsequent stimulation, but this hyporeactivity was reversible. Spreading of monocytes was rapidly aborted by the addition of this inhibitory factor. Thus, the inhibitory factor appeared to affect monocyte itself, but its effect seemed to be transient.